One way to make 0.1 M tricine buffer at a desired pH is to first make the following two stock solutions and then mix them together in different ratios. (10 pts)
Solution A: 0.1 M sodium tricine (dissolving produces Na+ Tricine0)
Solution B: 0.1 M disodium tricine (dissolving produces 2Na+ Tricine�1)
H2 Tricine+1 ? H+ + H Tricine0 pK1 = 2.3
H Tricine0 ? H+ + Tricine�1 pK2 = 8.2
What volumes of Solution A and Solution B should be mixed together to make 1 L of 0.1 M glycine buffer with a pH of 7.5? Be sure to include units.
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