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Question pls help and explain Spectrophotometric determinati


pls help and explain
Spectrophotometric determination of nitrite
Specific guidelines for practical report
Title: A brief description of the experiment.
Aim: A concise statement describing the objective of the experiment.
In your words describe the relevant theory for the experiment, using balanced equations where applicable. DO NOT simply reproduce what is in the laboratory manual; rather demonstrate that you have an understanding of the relevant background applicable to this experiment. This should not exceed half a page.
Materials and procedure
Generally it is sufficient to state �as per laboratory manual, p.xx�, unless authorised changes have been made. In this case details of the changes should be described.
Experimental data
Dilution of test sample: Record details of the dilution of test sample.
Visual observation: Record the visual appearance of assay samples.
Analytical data: Record the absorbance of each solution at 540 nm. The suggested format for a table to record this data is detailed below (remember to give the table a title):
Table �. (give title)
Nitrite Solution ( mg L-1) absorbance
0.00 0.0009
1.00 0.0858
2.00 0.1844
4.00 0.3736
Diluted sample 1 0.1753
Diluted sample 2 0.2326
Data interpretation and calculations
Comment on the visual interpretation of the colour of the diluted sample as compared to the standards.
Plot a standard curve using the absorbance values (y axis) and nitrite concentrations (x axis) for the blank and standards. The graph must be hand drawn on graph paper.
Using the standard curve, estimate the nitrite level in the diluted test samples (show lines of interpolation).
Calculate the nitrite level in the undiluted sample taking into account the dilution factor.
Calculate a relative spread for the absorbance values of the two diluted samples to indicate the precision of your test.
Clearly state the concentration of nitrite in the unknown sample – be sure to use appropriate units.
Provide a brief discussion of the results obtained, including possible sources of error in the experiment and how these may affect the results.
Discuss techniques of interpolation and extrapolation. Which of these is more accurate?
Explain why the test sample needed to be diluted.
Based on your calculated concentration of nitrite in the sample, is the water suitable for drinking?
Is your calculated nitrite level comparable to the expected value for this sample?

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