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The subject topic is bioinformatics. I need to compare TatC subunit from gram-positive and gram-negative bacteria in order to investigate differences in amino acid sequences and structure.
The gram positive bacteria that need to be used are:
Streptomyces scabies WP_059082011.1, Corynebacterium uterequi AKK11079.1, Corynebacterium glutamicum AMA00156.1, Staphylococcus aureus KFA45045.1, Bacillus amyloliquefaciens KDN92708.1 and Bacillus subtilis KFK77267.1.
The Gram-negative bacteria are; Aquifex aerolicus WP_010880807.1, Stenotrophomonas maltophilia AIL06264.1, Escherichia coli CCJ46466.1, Rickettsia prowazekii AMS12659.1, Rhizobium leguminosarum KZA97414.1 and Shewanella oneidensis WP_011073887.1
The databases from which i need the information from are:
Clustal Omega,
CLustal W2,
Phylogenetic tree,
Swiss model,
protter 1.0
My hypothesis is:
Gram-positive and Gram-negative bacteria have substantial structural and regional differentiation in TatC subunits and both of them contain different properties.
Aim is:
Whether this study was able to distinguish both the TatC regions and TatC subjects from Gram negative and Gram positive TatC bacteria.
Objectives include:
To choose up to six Gram negative TatC bacteria and six Gram positive TatC bacteria in order to compare and identify the amino acid sequences regions. (listed above).
? By means of several bioinformatics software tools such as BLAST to determine if the sequences were conservative or non-conservative.
? Whether the residues displayed polarity or non-polarity, also if they were positively charged or negatively charged.
? Finally, compare the structure, characteristics and properties of both Gram positive and Gram negative bacteria as well as in what way these exist different and how they are similar to one another.

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