BS6002 Advanced Blood Science
Assessed practical
Scenario.
You and four graduates have volunteered to work as bms as part a Médecins Sans Frontières relief operation. The aim of this operation is to provide basic medical care to the refugees in the Gevgelija refugee camp, Macedonia.
Due to the remote nature of the relief attempt funding is limited, transport and logistical support is minimal, meaning no additional laboratory equipment can be provided for the near future. Turkey is currently supplying blood on a 24 hour notice period for specific patients.
Currently, your tented laboratory is operating on a diesel generator which provides power only for three hours. All investigations must be complete within this time frame.
If justified, you are required to order transfusion products.
You are required to investigate two patients from the refugee camp. The result of the investigation should be comparable with your diagnostic conclusion.
Patient A is a 3 y/o female Syrian national who presented with symptoms of fatige, malaise and has failed to thrive. Clinical presentation showed an underdeveloped and underweight female whom shows mucosal pallor and frontal bossing. Further clinical questioning revealed that the bossing was never investigated as this is a common trait in the maternal family.
Patient B is a 14 y/o male Syrian national. The boy presented as an emergency case 20 minutes ago, with a large laceration which has severed his femoral vein. Emergency intervention sutured the vascular tissue and arrested the bleeding by surgical means. The cause of the injury is unclear, no witness or family member has come forward and the boy remains unconscious.
Prioritise your patients and plan accordingly.
Available Equipment
Microscope, with x10, x40 and x100(oil) objectives
Microscope slides and coverslips, immersion oil
Haematocrit centrifuge
Glass capillary tubes
Gel electrophoresis tank (horizontal) and power pack
Micropipettes (2-20, 20-200, 200-1000 ml) and tips
Weighing balance
Stopwatches
Some glassware/plasticware, including measuring cylinders, conical flasks, beakers, various-sized test tubes, plastic Eppendorf tubes, plastic pastettes
Chemicals, Reagents, Stains:
Agarose
Boric acid
Blood Grouping reagents
Dextrose (Glucose)
Disodium EDTA
Prothombin kit
Glycerol
Methylene Blue dye (powder)
Nile Blue Sulphate dye (powder)
Sodium chloride
Sodium nitrite
Speedy diff
Potassium dihydrogen phosphate
Di-Potassium hydrogen phosphate
Tris base
TBE buffer solution (concentrate)
Urea
Assessment.
You are required to select investigations, appropriate to the patient’s conditions and carry them out. You are also required to justify the prioritisation of your investigations and order blood if appropriate.
To evidence the above process, you are required to write up your practical in the style suitable to publish either in a journal called ‘Biochemistry’ for their ‘Rapid Reports’ section – an example of which is posted here, or to publish in the journal ‘Nature’, in their ‘Letters’ section – example posted here. (2000 word excluding supporting information section)
In your report, you are required to address the following points:
A brief description of the case
Rationale for choice of tests used and the priority assigned to each
Appropriateness of those available tests
A very brief discussion of other tests that might have been equally (or even more) appropriate, had they been available.
Analysis of the results, in terms of case diagnosis, with supporting results.
Further investigations that might be appropriate.
The Report to be fully referenced throughout.
Format:
Extracted and adapted from the Biochemistry authors guide (items in red are our instructions)
Type set: Main body – Font (Arial 12, Times New Roman 12, or Calibri 12), double spacing, Legends – Font (Arial 10, Times New Roman 10, or Calibri 10), single spacing
Preparation of Rapid Reports/Letters
Title, author, and affiliations. Provide contact information for the corresponding author ie. an email address.
Abstract (no more than 150 words)
Keywords
Main Text
Includes an introduction, a brief description of experimental procedures, and the results and discussion in one combined block of text (no headings for subsections)
Display Items such as tables, structures, charts, schemes, figures, and figure legends should be embedded in the Main Text.
Detailed Experimental Procedures should be included in a separate ‘Supporting Information’ section. These methods should be described in sufficient detail to enable others to repeat the experiment.
References:
Note that for Rapid Reports/Letters the inclusion of complete titles for the references is optional; however, complete titles should be included if space permits. (For this coursework complete titles are required). Authors to given in full, NOT et al. in reference list.
Acknowledgements: Include here the LondonMet Student ID Numbers of the other members of your group who worked together.
Supporting Information:
Detailed methodologies used with suitable justification of investigations (include in the main file, NOT in a separate file)
Manuscript Assembly:
Title Page
Abbreviations and Textual Footnotes
Abstract
Introduction (no heading)
Experimental Procedures (Materials and Methods – in summary)
Results
Discussion
Acknowledgements
Supporting Information section
References
Tables – embedded in the main text
Structures, Charts, Schemes
Figure Legends
Figures
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