100% Original, Plagiarism Free, Tailored to your instructions

Order Now!

The inactivation curve is done by plotting temperature against time

The inactivation curve is done by plotting temperature against time (Holger 2002, p. 47). The area under the curve is then determined using the
trapezoid rule. Afterwards the D – value of the thermal processes can be determined (Yoshihiro, Eugene and Ludwig 2002, 250).
For all the graphs, X axis are the plot for temperature whereas y axis represents time in seconds.

Inactivation Curves for each Set of Results


Explanation for the shapes of the inactivation curves

For all the curves, the temperature increases as the time for inoculation increase at the
start. This is a clear indication that the bacteria survived the low temperatures. However, the situation changes after the temperature reaches 1 degree as the temperature increase but the time decrease for the Streptococcus faecalis and E.Coli while that of Staphylococcus aureus changes at 2 degree temperature.
This indicates that the bacteria became inactive and succumbed to the high temperatures.

Appropriate data to determine the D values* for each organism and reasons for the choice

The data selected for the three vegetative bacteria is from time 0 to time 5 minutes because using logarithm to base 10, the data becomes linear, and would be more representative of the inactivation process. The units for the inactivation rate are colony forming units per ml per minute.

Determination of D values* for each organism and comments

For Staphylococcus aureus the D values* are:
D values* at 5oC
D values* at 20oC = 1.0852
D values* at 35oC = 1.1677
The D values* for Streptococcus feacalis are:
D values * at 5oC
D values* at 20oC = 1.2056
D values* at 35oC = 1.1685
The D values* for E. coli are:
D values* at 5oC
D values * at 20oC = 1.1167
D values * at 35oC = 1.1361
It can be concluded that for Staphylococcus aureus, the highest inactivation rate was 1.1677cfu/ml/minute at 35oC, whereas the lowest was at 5oC.
The highest inactivation rate for Streptococcus feacalis was 1.2056cfu/ml/minute at 20oC and the lowest was at 5oC.
The highest inactivation rate for E.coli was 1.1361cfu/ml/minute at 35oC, and the lowest rate was at 5oC.
In conclusion, as the temperature increased the rate of inactivation increased, and for Staphylococcus aureus and E.coli, 35oC
was the optimum temperature for inactivation by thermal-chemical disinfection.

Our Service Charter

  1. Excellent Quality / 100% Plagiarism-Free

    We employ a number of measures to ensure top quality essays. The papers go through a system of quality control prior to delivery. We run plagiarism checks on each paper to ensure that they will be 100% plagiarism-free. So, only clean copies hit customers’ emails. We also never resell the papers completed by our writers. So, once it is checked using a plagiarism checker, the paper will be unique. Speaking of the academic writing standards, we will stick to the assignment brief given by the customer and assign the perfect writer. By saying “the perfect writer” we mean the one having an academic degree in the customer’s study field and positive feedback from other customers.
  2. Free Revisions

    We keep the quality bar of all papers high. But in case you need some extra brilliance to the paper, here’s what to do. First of all, you can choose a top writer. It means that we will assign an expert with a degree in your subject. And secondly, you can rely on our editing services. Our editors will revise your papers, checking whether or not they comply with high standards of academic writing. In addition, editing entails adjusting content if it’s off the topic, adding more sources, refining the language style, and making sure the referencing style is followed.
  3. Confidentiality / 100% No Disclosure

    We make sure that clients’ personal data remains confidential and is not exploited for any purposes beyond those related to our services. We only ask you to provide us with the information that is required to produce the paper according to your writing needs. Please note that the payment info is protected as well. Feel free to refer to the support team for more information about our payment methods. The fact that you used our service is kept secret due to the advanced security standards. So, you can be sure that no one will find out that you got a paper from our writing service.
  4. Money Back Guarantee

    If the writer doesn’t address all the questions on your assignment brief or the delivered paper appears to be off the topic, you can ask for a refund. Or, if it is applicable, you can opt in for free revision within 14-30 days, depending on your paper’s length. The revision or refund request should be sent within 14 days after delivery. The customer gets 100% money-back in case they haven't downloaded the paper. All approved refunds will be returned to the customer’s credit card or Bonus Balance in a form of store credit. Take a note that we will send an extra compensation if the customers goes with a store credit.
  5. 24/7 Customer Support

    We have a support team working 24/7 ready to give your issue concerning the order their immediate attention. If you have any questions about the ordering process, communication with the writer, payment options, feel free to join live chat. Be sure to get a fast response. They can also give you the exact price quote, taking into account the timing, desired academic level of the paper, and the number of pages.

Excellent Quality
Zero Plagiarism
Expert Writers

Instant Quote

Single spaced
approx 275 words per page
Urgency (Less urgent, less costly):
Total Cost: NaN

Get 10% Off on your 1st order!