The inactivation curve is done by plotting temperature against time (Holger 2002, p. 47). The area under the curve is then determined using the
trapezoid rule. Afterwards the D – value of the thermal processes can be determined (Yoshihiro, Eugene and Ludwig 2002, 250).
For all the graphs, X axis are the plot for temperature whereas y axis represents time in seconds.
Inactivation Curves for each Set of Results
Explanation for the shapes of the inactivation curves
For all the curves, the temperature increases as the time for inoculation increase at the
start. This is a clear indication that the bacteria survived the low temperatures. However, the situation changes after the temperature reaches 1 degree as the temperature increase but the time decrease for the Streptococcus faecalis and E.Coli while that of Staphylococcus aureus changes at 2 degree temperature.
This indicates that the bacteria became inactive and succumbed to the high temperatures.
Appropriate data to determine the D values* for each organism and reasons for the choice
The data selected for the three vegetative bacteria is from time 0 to time 5 minutes because using logarithm to base 10, the data becomes linear, and would be more representative of the inactivation process. The units for the inactivation rate are colony forming units per ml per minute.
Determination of D values* for each organism and comments
For Staphylococcus aureus the D values* are:
D values* at 5oC
D values* at 20oC = 1.0852
D values* at 35oC = 1.1677
The D values* for Streptococcus feacalis are:
D values * at 5oC
D values* at 20oC = 1.2056
D values* at 35oC = 1.1685
The D values* for E. coli are:
D values* at 5oC
D values * at 20oC = 1.1167
D values * at 35oC = 1.1361
It can be concluded that for Staphylococcus aureus, the highest inactivation rate was 1.1677cfu/ml/minute at 35oC, whereas the lowest was at 5oC.
The highest inactivation rate for Streptococcus feacalis was 1.2056cfu/ml/minute at 20oC and the lowest was at 5oC.
The highest inactivation rate for E.coli was 1.1361cfu/ml/minute at 35oC, and the lowest rate was at 5oC.
In conclusion, as the temperature increased the rate of inactivation increased, and for Staphylococcus aureus and E.coli, 35oC
was the optimum temperature for inactivation by thermal-chemical disinfection.
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