I need to explain procedure from the data I submitted and
The staining procedure for the Hematoxylin and Eosin involved four stages. These stages are removal of paraffin, rehydration, staining and dehydration. Removal of paraffin involves mixing Xylene or Neo-Clear for 3 minutes. It also involves another Xylene or Neo-Clear for 2 minutes. The second stage is the rehydration which involves adding 100% ErOH, 100% ErOH for 1 minute, and 95% ErOH for 1 minute and water for 3 minutes. The staining stage involves mixing Hematoxylin for 2 minutes, and water wash for 2-5 minutes in running water. This stage also involves adding scott solution to blue hematoxylin for 2 minutes. Eosin is also added 1-3 minutes, and this is checked after 1 minute.
The last is the dehydration which involves adding 1 or 2 dips of 70% ErOH, 1 or 2 dips of 70% ErOH, 1 or 2 dips of 95% ErOH and 1 or 2 dips of 95% ErOH. The rinses are performed quickly to preserve the intensity of the cosin counterstain. After rinsing, 100% ErOH is mixed for 3 minutes twice. In addition, Xylene or Neo-Clear is added for 3 minutes twice. After the four stages are done, the specimen is mounted with Cytoseal XYL and covered with a coverslip before the sample dries.
explain difference between reported method and actual method followed.